Nikon BioImaging Lab USA

🌟 See how the #NikonBioImagingLabUSA can apply #SpatialBiology tools to uncover new insights in #CancerBiology. 📄 In this poster we presented at the 2024 Society for Immunotherapy of Cancer (SITC) conference, we detail a multimodal approach for imaging and analysis of immune cell markers, immune checkpoint blockade markers, and structural markers in a non-small cell lung cancer #NSCLC patient sample. 🔬 With this method, one section is used for multiplex immunofluorescence with the #LunaphoreCOMET system, traditional H&E staining/imaging, and second harmonic generation #SHG #Imaging with the Nikon AX R MP #Multiphoton #Microscopy system. An adjacent section is used for #SpatialTranscriptomics with the 10x Genomics Visium CytAssist platform, allowing for comparison of gene and protein expression. 🔎 One of our key findings was a clear distribution of VISTA and IDO1-enriched tissue regions. 🔗 NBIL - Your spatial biology partner: https://bit.ly/41OJEzi #Lunaphore #MultiplexIF Lunaphore (a Bio-Techne brand)

🌟 Exciting News! 🌟 🧑🔬🔬 We’re thrilled to introduce the Nikon BioImaging Lab Boston, your gateway to cutting-edge #Imaging solutions for the #LifeSciences and beyond! 📍 Located in Greater Boston area, our state-of-the-art facility offers: ✨ Advanced #Microscopy technologies ✨ Expert imaging support for your research ✨ High-quality data acquisition & analysis 🚀 Whether you're exploring topics in #CellBiology, #DrugDiscovery, or other innovative fields, our team is here to help you achieve breakthrough results. 🔗 Ready to elevate your research? Visit us to learn more and schedule your imaging session today: https://bit.ly/3E3YqKv #NikonBioImagingLabUSA #Microscope #NBIL #NikonMicroscopy #SpatialBiology #HighContentImaging

NSCLC post 2/n 🔬 We imaged a non-small cell lung cancer #NSCLC sample prepared using a 16-plex antibody panel on the #LunaphoreCOMET system at the #NikonBioImagingLabUSA. We aimed to visualize immune checkpoint blockade markers and immune cell markers in an NSCLC sample, along with some structural markers. 📖 What did we learn from the expression of immune checkpoint and other markers in NSCLC? ▶️ Apart from the widely studied immune checkpoint inhibitors (ICI) PD-1 and PD-L1 there is expression of other ICI markers. ▶️ VISTA and IDO1 are expressed in spatially distinct regions within the tissue. IDO1 is expressed on a subpopulation of PanCK+ epithelial cells, and its intensity varies across the epithelial cell population in which it's expressed. ▶️ PD-L1 is expressed on CD4+ T cells and PanCK+ epithelial cells. The PD-L1 intensity on T cells was 2.5-fold higher than on epithelial cells. ▶️ Natural Killer cells interact with VISTA+ positive cells. ▶️ Extensive remodeling of the lung tissue during NSCLC invasion, as seen by expression of a-SMA. ▶️ Presence of immature and mature tertiary lymphoid structures (TLS). 🛑 Immune checkpoint blockade markers PD-1: Helps in regulating immune responses and maintaining immune tolerance. It is expressed on T cells, B cells, monocytes, and dendritic cells. Pembrolizumab and Nivolumab are PD-1 inhibitors approved for NSCLC. PD-L1: Helps in regulating immune responses and maintaining immune tolerance. It is expressed on CD4 T cells. It is upregulated in epithelial cells during EMT, often associated with cancer progression. Durvalumab is a PD-L1 inhibitor approved for NSCLC. IDO1: Expressed in tumor cells in NSCLC, leading to an immunosuppressive tumor microenvironment, and myeloid cells. Various inhibitors are in clinical trials for use in combination with PD-1 inhibitors or other therapies. VISTA: Expressed in tumor cells in NSCLC, leading to an immunosuppressive tumor microenvironment, myeloid cells, T cells, and tumor-infiltrating lymphocytes. Other checkpoint blockade markers that were not included in this study but could be further explored: CTLA-4 LAG-3 TIM-3 TIGIT 🔗 NBIL - Your spatial biology partner: https://bit.ly/41OJEzi #Lunaphore #MultiplexIF #SpatialBiology #CancerBiology #ICI #CheckpointBlockade Lunaphore (a Bio-Techne brand)

⌛ Last day to meet us at #USCAP2025! 🔬 Stop by booth 700 for information about the #NikonBioImagingLabUSA and the #ContractResearch #CRO services we offer – supporting research applications in #Pathology and #Histology. 🔗 Click for more information: https://bit.ly/4hJU88Z #NikonMicroscopy #Microscopy #Imaging #DigitalPathology #SpatialBiology

🔬 We’re having a great time at #USCAP2025 👋 Meet us at booth 700 to learn about the #NikonBioImagingLabUSA and the variety of #ContractResearch #CRO services we offer for applications in #Pathology, including chromogen imaging and analysis for #Histopathology. 🔗 Learn more about what we can do for your research: https://bit.ly/4hJU88Z #NikonMicroscopy #Microscopy #Imaging #DigitalPathology #SpatialBiology

🤔 Thinking beyond DAB for your #Immunohistochemistry and #InSituHybridization assays? 🌟 The #NikonBioImagingLabUSA makes #HighContent multiplexed imaging of next-generation chromogens easy using the NIS-Elements software. #Brightfield and #Fluorescence #Microscopy can be simply combined in the same automated experiment. 🤖 #AI makes it possible to quickly perform critical image analysis tasks (e.g., segmentation) and obtain important quantitative insights, such as the number of immune cells in a certain tissue region. Want to learn more? Drop by for an in-person discussion at #USCAP2025, booth 700. 🔗 You can learn more about the types of applications we support here: https://bit.ly/4iPxJbo #NikonMicroscopy #Imaging #Pathology #Histology #SpatialBiology

NSCLC Post 1/n 🔬 The video below features data from a non-small cell lung cancer #NSCLC sample prepared with a 16-plex antibody panel and imaged using the #Lunaphore COMET system at the #NikonBioImagingLabUSA. 👀 We visualized a range of immune cell markers, immune checkpoint blockade markers, and structural markers. 💪 Immune Cell Markers: CD3: Expressed on all T cells CD4: Helper T cells that interact with antigen presenting cells and help activate other immune cells CD8: Cytotoxic T cells or Killer T cells. They identify and interact with infected or abnormal cells releasing Granzyme B to induce apoptosis or programmed cell death CD20: Expressed on B cells CD56: Natural Killer (NK) cells Granzyme B: Present in NK cells and cytotoxic T cells. These cells make contact with and release Granzyme B granules in diseased cells, initiating cell death 🚧 Structural markers: CD31/PECAM-1: Expressed in endothelial cells lining blood vessels and immune cells a-SMA: Expressed by myofibroblasts and smooth muscle cells. In cancer, it's highly expressed in myofibroblasts playing a role in tissue remodeling PanCK: Detects multiple cytokeratins expressed in epithelial cells E-Cadherin: Cell adhesion molecule primarily expressed in epithelial cells 🛑 Immune checkpoint blockade markers PD-1: Helps regulate immune responses and maintain immune tolerance. It's expressed on T cells, B cells, monocytes, and dendritic cells PD-L1: Helps regulate immune responses and maintain immune tolerance. It's expressed on CD4 T cells. It's upregulated in epithelial cells during EMT - often associated with cancer progression VISTA: Expressed in NSCLC tumor cells, leading to an immunosuppressive tumor microenvironment, myeloid cells, T cells, and tumor-infiltrating lymphocytes IDO-1: Expressed in NSCLC tumor cells, leading to an immunosuppressive tumor microenvironment and myeloid cells 🔳 Other markers: Ki-67: Proliferating cells Cleaved Caspase- 3: Cells undergoing apoptosis or programmed cell death 🔗 NBIL - Your spatial biology partner: https://bit.ly/41OJEzi #LunaphoreCOMET #MultiplexIF #SpatialBiology #CancerBiology Lunaphore (a Bio-Techne brand)

🤝 Meet us at #USCAP2025 starting Monday, March 24th at booth 700! We look forward to discussing our contract research #CRO imaging and analysis services for #Pathology. 🔗 See our website for more details: https://bit.ly/4ix6fY5 #NikonBioImagingLabUSA #NikonMicroscopy #Microscopy #Microscope #WholeSlideImaging #DigitalPathology #SpatialBiology

🎉 We’re on LinkedIn! 🎉 Welcome to the official account of the Nikon BioImaging Lab USA! 🌟 At our facility, we combine advanced #Microscopy technologies with expert support to bring your research to life. From #CellBiology to #DrugDiscovery and beyond, we’re here to be your trusted imaging partner every step of the way. 🧬🔬 Check out the video below to learn more! We’re not just another lab—we’re an extension of your team. Let us handle the imaging, so you can focus on what you do best: pushing the boundaries of science. 🚀 📍 Located in Boston, USA 🔗 Learn more: https://bit.ly/4bGCxNY #NikonBioImagingLabUSA #WelcomePost #LifeSciences #CRO