Ali Hussain

Ali Hussain

Irvine, California, United States
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NJIT / UMDNJ

About

Development of Innovative Medical Devices.
Business Model Development
Strategic…

Activity

Experience

Education

  • NJIT / UMDNJ

    Activities and Societies: Graduate Biomedical Engineering Society Biomaterials / Tissue Engineering Weekly Discussion Group

    Dissertation Title: Design and Development of 3-Dimensional Vascularized Tri-culture Cardiac Constructs Using Chitosan Nanofiber Scaffolds

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    Activities and Societies: 2 industry internships at Pfizer Global Research & Devlopment, Groton, CT.

    Concetration: Tissue Engineering and Biomaterials

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    Activities and Societies: Honors Society, American Chemical Scoiety

    Awarded scholarship to participate in travel abroad program to study junior year in Manchester, United Kingdom.

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    Activities and Societies: Travel Club, Canoeing and Kayaking team.

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Publications

  • Development and In vitro Evaluation of Infection Resistant Materials: A Novel Surface Modification Process for Silicone and Dacron

    Journal of Biomaterials Applications

    Silicone and Dacron are used in a wide spectrum of implantable and indwelling medical products. They elicit a foreign body response, which results in a chronic inflammatory environment and collagenous encapsulation of the medical device that compromises the immune system’s ability to effectively fight infections at the biomaterial surface. The objective of this work is to evaluate a novel process to modify silicone and Dacron with a bioactive collagen surface coupled to a gentamicin impregnated…

    Silicone and Dacron are used in a wide spectrum of implantable and indwelling medical products. They elicit a foreign body response, which results in a chronic inflammatory environment and collagenous encapsulation of the medical device that compromises the immune system’s ability to effectively fight infections at the biomaterial surface. The objective of this work is to evaluate a novel process to modify silicone and Dacron with a bioactive collagen surface coupled to a gentamicin impregnated hydrogel graft and assess the surface’s cytocompatibility and infection resistance properties. Samples of silicone and polyethylene terephthalate (Dacron velour) were modified by plasma deposition and activation followed by a co-polymer acrylic acid (AA) / acrylamide (AAm) hydrogel graft and covalent immobilization of a bioactive collagen surface. The modified surfaces were characterized using FTIR, contact angle, staining, SEM, and XPS. The Poly (AA-AAm) hydrogel was impregnated with gentamicin and tested for controlled release characteristics. Each modified surface was evaluated for its ability to resist infection and to promote normal healing as measured by bacterial growth inhibition (Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa) in both broth and agar conditions as well as using fluorescence microscopy to observe adherence of 3T3-NIH fibroblasts. The addition of the Poly (AA-AAm) hydrogel with gentamicin inhibited bacterial growth and the subsequent addition of the collagen surface promoted robust fibroblast adhesion on both silicone and Dacron materials. Thorough surface characterization and in vitro bacterial and fibroblast evaluation results suggest this novel surface bioengineering process generated a highly effective surface on silicone and Dacron with the potential to reduce infection and promote healing.

    Other authors
    See publication

  • Effects of surface-bound and intravenously administered heparin on cell-surface interactions: inflammation and coagulation

    Perfusion

    Intravenous administration of heparin and heparin-bonded extracorporeal circuits are frequently used to mitigate the deleterious effects of blood contact with synthetic materials. The work described here utilized human blood in a micro-perfusion circuit to experimentally examine the effects of intravenous and surface-bound heparin on cellular activation. Activation markers of coagulation and of the inflammatory response were examined using flow cytometry; specifically, markers of platelet…

    Intravenous administration of heparin and heparin-bonded extracorporeal circuits are frequently used to mitigate the deleterious effects of blood contact with synthetic materials. The work described here utilized human blood in a micro-perfusion circuit to experimentally examine the effects of intravenous and surface-bound heparin on cellular activation. Activation markers of coagulation and of the inflammatory response were examined using flow cytometry; specifically, markers of platelet, monocyte, polymorphonuclear leukocyte (PMN), and lymphocyte activation were quantified. The results indicate that surface-bound heparin reduces the inflammatory response whereas systemically administered heparin does not. This finding has important implications for blood-contacting devices, particularly within the context of recently elucidated connections between inflammation pathways and coagulation disorders. Data presented indicate that surface-bound heparin and intravenously administered heparin play distinct, but vital roles in rendering biomaterial surfaces compatible with blood

    Other authors
    • Greg Johnson
    • Ben Curry
    • Linda Cahalan
    • Patrick Cahalan
    • Mark Gartner
    • Roni Prater
    • John Biggerstaff
    See publication

  • Adhesive-tape soft lithography for patterning mammalian cells: application to wound-healing assays

    BioTechniques

    This paper introduces a benchtop method for patterning mammalian cells that does not require access to photolithographic capabilities. This paper shows how cells can be patterned easily with sub-millimeter precision using a non-photolithographic technique that is based on the use of office adhesive tape and PDMS. This method is fast, biocompatible, reliable, safe, inexpensive, and suitable for biomedical researchers, as it only requires materials and tools commonly found in a biomedical…

    This paper introduces a benchtop method for patterning mammalian cells that does not require access to photolithographic capabilities. This paper shows how cells can be patterned easily with sub-millimeter precision using a non-photolithographic technique that is based on the use of office adhesive tape and PDMS. This method is fast, biocompatible, reliable, safe, inexpensive, and suitable for biomedical researchers, as it only requires materials and tools commonly found in a biomedical laboratory. We believe this tape-based soft lithography can empower biologically oriented researchers to produce their own microfluidic devices, freeing them from the need to use a clean room.

    Other authors
    • Anil B. Shrirao
    • Cheul H. Cho
    • Raquel Perez-Castillejos
    See publication

  • Functional 3-D cardiac co-culture model using bioactive chitosan nanofiber scaffolds

    Biotechnology and Bioengineering

    The in vitro generation of a three-dimensional (3-D) myocardial tissue-like construct employing cells, biomaterials, and biomolecules is a promising strategy in cardiac tissue regeneration, drug testing, and tissue engineering applications. The objective of this study was to fabricate bioactive 3-D chitosan nanofiber scaffolds using an electrospinning technique and exploring its potential for long-term cardiac function in the 3-D co-culture model. Chitosan is a natural polysaccharide…

    The in vitro generation of a three-dimensional (3-D) myocardial tissue-like construct employing cells, biomaterials, and biomolecules is a promising strategy in cardiac tissue regeneration, drug testing, and tissue engineering applications. The objective of this study was to fabricate bioactive 3-D chitosan nanofiber scaffolds using an electrospinning technique and exploring its potential for long-term cardiac function in the 3-D co-culture model. Chitosan is a natural polysaccharide biomaterial that is biocompatible, biodegradable, non-toxic, and cost effective. Electrospun chitosan was utilized to provide structural scaffolding characterized by scale and architectural resemblance to the extracellular matrix (ECM) in vivo. The chitosan fibers were coated with fibronectin via adsorption in order to enhance cellular adhesion to the fibers and migration into the interfibrous milieu. Ventricular cardiomyocytes were harvested from neonatal rats and studied in various culture conditions (i.e., mono- and co-cultures) for their viability and function. The results demonstrate that the chitosan nanofibers retained their cylindrical morphology in long-term cell cultures and exhibited good cellular attachment and spreading in the presence of adhesion molecule, fibronectin. Cardiomyocyte mono-cultures resulted in loss of cardiomyocyte polarity and islands of non-coherent contractions. However, the cardiomyocyte-fibroblast co-cultures resulted in polarized cardiomyocyte morphology and retained their morphology and function for long-term culture. The Cx43 expression in the fibroblast co-culture was higher than the cardiomyocytes mono-culture and endothelial cells co-culture. In addition, fibroblast co-cultures demonstrated synchronized contractions involving large tissue-like cellular networks. To our knowledge, this is the first attempt to test chitosan nanofiber scaffolds as a 3-D cardiac co-culture model.

    Other authors
    • George Collins
    • Derek Yip
    • Cheul Cho
    See publication

  • Electrospun chitosan-based nanofiber scaffolds for cardiac tissue engineering applications

    Bioengineering Conference, Proceedings of the 2010 IEEE 36th Annual Northeast

    The objective of this study was to fabricate 3-dimensional (3D) chitosan nanofiber scaffolds using an electrospinning technique and explore its potential for cardiac tissue engineering. Three culture conditions were tested: cardiomyocytes only, cardiomyocytes-fibroblasts cocultures, and cardiomyocyte-endothelial cells cocultures. The cells were seeded on 2-dimensional (2D) chitosan films and 3D chitosan nanofibers. Cellular morphology and functionality were assessed using immunofluorescent…

    The objective of this study was to fabricate 3-dimensional (3D) chitosan nanofiber scaffolds using an electrospinning technique and explore its potential for cardiac tissue engineering. Three culture conditions were tested: cardiomyocytes only, cardiomyocytes-fibroblasts cocultures, and cardiomyocyte-endothelial cells cocultures. The cells were seeded on 2-dimensional (2D) chitosan films and 3D chitosan nanofibers. Cellular morphology and functionality were assessed using immunofluorescent staining for alpha-sarcomeric actin (SA) and gap junction protein, connexin-43 (Cx43). In both the 2D and 3D scaffolds, only the cardiomyocyte-fibroblasts cocultures resulted in polarized cardiomyocyte morphology, fibril SA expression and Cx43 expression was higher compared to the other two conditions. In addition, the fibroblasts cocultures demonstrated synchronized contractions involving large tissue-like cellular networks. To our knowledge, this is the first attempt to utilize 3D chitosan nanofibers as cardiomyocyte scaffolds. Our results demonstrate that chitosan nanofibers can serve as a potential scaffold that can retain cardiomyocyte morphology and function.

    Other authors
    • George Collins
    • Cheul Cho
    See publication

Patents

  • Methods of making bioactive collagen medical scaffolds such as for wound care dressings, hernia repair prosthetics, and surgical incision closure members

    Issued US US 14/041,372

    A method of preparing a crosslinked, collagen-based medical scaffold is provided, comprising: (a) immersing a sample of fibrous and/or non-fibrous collagen in a buffered acidic, aqueous solution comprising an alcohol; (b) contacting the collagen in solution with a catalytic component comprising 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride for a time at least sufficient to effect reaction between amino and carboxyl groups present on the collagen and to yield crosslinked collagen…

    A method of preparing a crosslinked, collagen-based medical scaffold is provided, comprising: (a) immersing a sample of fibrous and/or non-fibrous collagen in a buffered acidic, aqueous solution comprising an alcohol; (b) contacting the collagen in solution with a catalytic component comprising 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride for a time at least sufficient to effect reaction between amino and carboxyl groups present on the collagen and to yield crosslinked collagen that is resistant to pronase degradation; and (c) drying the crosslinked collagen to yield a porous, crosslinked collagen article wherein the porous, crosslinked collagen article demonstrates a pore size of 10-500 microns. Also provided are bioactive collagen medical scaffolds for wound care dressings, hernia repair prosthetics, and surgical incision closure members, prepared using the method above

    See patent

  • Surface treated staples, sutures and dental floss and methods of manufacturing the same

    Filed US US 20140288592 A1

    A variety of medical devices including staples sutures and dental floss with surface treatment on at least one tissue-facing surface to improve biologic function such as to control tissue adhesion are disclosed including heparin surface treatment which provides heparin present in an amount to yield heparin bioactivity of at least one of i) an ATIII binding of at least 2 pmol/cm2, and ii) a thrombin deactivation of at least 0.2 IU/cm2; an acrylic surface treatment for coupling thereto of a…

    A variety of medical devices including staples sutures and dental floss with surface treatment on at least one tissue-facing surface to improve biologic function such as to control tissue adhesion are disclosed including heparin surface treatment which provides heparin present in an amount to yield heparin bioactivity of at least one of i) an ATIII binding of at least 2 pmol/cm2, and ii) a thrombin deactivation of at least 0.2 IU/cm2; an acrylic surface treatment for coupling thereto of a heparin surface treatment, a collagen surface treatment or both; and an amino-functional polysiloxane surface treatment for coupling thereto of a heparin surface treatment.

    See patent

  • Surface Treated Polymeric Synthetic Hernia Mesh Prosthesis, Surface Treated Sutures and Staples and Methods of Manufacturing the Same

    Filed US US20130110137 A1

    A variety of polymeric synthetic hernia mesh prosthesis with surface treatment on at least one tissue-facing surface to control tissue adhesion are disclosed including heparin surface treatment which provides heparin present in an amount to yield heparin bioactivity of at least one of i) an ATIII binding of at least 2 pmol/cm.sup.2, and ii) a thrombin deactivation of at least 0.2 IU/cm.sup.2; an acrylic surface treatment for coupling thereto of a heparin surface treatment, a collagen surface…

    A variety of polymeric synthetic hernia mesh prosthesis with surface treatment on at least one tissue-facing surface to control tissue adhesion are disclosed including heparin surface treatment which provides heparin present in an amount to yield heparin bioactivity of at least one of i) an ATIII binding of at least 2 pmol/cm.sup.2, and ii) a thrombin deactivation of at least 0.2 IU/cm.sup.2; an acrylic surface treatment for coupling thereto of a heparin surface treatment, a collagen surface treatment or both; and an amino-functional polysiloxane surface treatment for coupling thereto of a heparin surface treatment. The synthetic hernia mesh may be formed of monofilament or multifilament polypropylene or polyester, and may be formed as a multi-layer prosthesis with an outer layer formed of a polymeric synthetic hernia mesh with surface treatment to control tissue adhesion coupled to one or more polymeric synthetic hernia meshes without such surface treatments.

    See patent

  • System and method for vascularized biomimetic 3-d tissue models

    Filed US WO 2012119012 A1

    The present invention relates to a vascularized three dimensional construct for thick tissue, a process for making the construct and to the use of the construct in tissue regeneration and repair and in drug development. The three-dimensional (3-D) tissue technology is used to generate vascularized, biomimetic tissue models in vitro utilizing a biodegradable nanofiber scaffold. The culture system allows the maintenance of long-term survival and function of liver and heart cells. The system…

    The present invention relates to a vascularized three dimensional construct for thick tissue, a process for making the construct and to the use of the construct in tissue regeneration and repair and in drug development. The three-dimensional (3-D) tissue technology is used to generate vascularized, biomimetic tissue models in vitro utilizing a biodegradable nanofiber scaffold. The culture system allows the maintenance of long-term survival and function of liver and heart cells. The system utilizes a novel approach to generate structures that mimic in vivo tissue architecture. The system provides a microenvironment for forming 3-D microvascular networks within the nanofiber scaffolds.

    Other inventors
    • Cheul Cho
    • George Collins
    See patent

Projects

Languages

  • English

    Native or bilingual proficiency

  • Arabic

    Native or bilingual proficiency

  • Persian

    Native or bilingual proficiency

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