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Indian Journal of Animal Research Published Volume 58 Issue 5 (May 2024) The Effect of Intrauterine Administration of Zeolite on the Puerperal Period in Dairy Cows O.V. Giurgiu, M.L. Bogdan, A. Ionescu, M.S. Ciupe, C.R. Cimpean, N. Hitter-Purcar, D.G. Bitica, L.M. Gherman, S. Bogdan, D.I. Berean doi: 10.18805/IJAR.BF-1730 Cite article:- Giurgiu O.V., Bogdan M.L., Ionescu A., Ciupe M.S., Cimpean C.R., Hitter-Purcar N., Bitica D.G., Gherman L.M., Bogdan S., Berean D.I. (2024). The Effect of Intrauterine Administration of Zeolite on the Puerperal Period in Dairy Cows . Indian Journal of Animal Research. 58(5): 746-752. doi: 10.18805/IJAR.BF-1730. ABSTRACT Background: The development of new protocols has become a priority today in the context of the adverse effects of conventional treatments. Zeolite falls into this category due to its numerous applications reported in the specialized literature. In this study, the main expected objectives were to demonstrate that intrauterine administration of zeolite can contribute to improving the indicators underlying the development of pathologies during the postpartum period. Methods: To determine the dosage and potential toxic effects on the uterine mucosa, we conducted a study on rats, to which we administered different doses on the second day after delivery. Ultrasound measurements of the uterus, collection of bacteriological samples and uterine tissue were performed at 7, 14 and 21 days. The results were satisfactory in terms of tissue effects, with the 20% dosage being the most suitable. Following the completion of the preliminary study, we grouped the animals into two equal groups (n=32). Intrauterine administration of zeolite was performed on the second day after delivery and monitoring of uterine involution was carried out on days 7, 14 and 21 of the puerperium. At 14 days, we collected samples to determine the number of bacteria and uterine tissue. Result: The data obtained indicate that the group receiving intrauterine zeolite (n=16) experienced a decrease in the number of bacteria, significantly faster uterine involution on days 14 and 28, a more favourable uterine score and a significantly shorter service period. However, no significance was established regarding the number of doses of seminal material used. KEYWORDS Puerperal Period, Uterine Environment, Zeolite https://lnkd.in/gWA-dYx2

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